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CORE FACILITIES

 

Electron Microscopy

 

High Content Screening Facility

 

Histology

 

Microscopy

 

Viral Vector Core Facility

 

 

Home > Research Research Interests > Viral Vector Core

 

 

 

VIRAL VECTOR CORE FACILITY

 

 

 

The Miami Project to Cure Paralysis
Lois Pope LIFE Center Building  Room 4-26A

 

 

Please email questions to:  VVC@umail.miami.edu

 

 

ABOUT THE VIRAL VECTOR CORE FACILITY

 

The Viral Vector Core (VVC) is open to all University of Miami researchers. The VVC specializes in producing lentiviral and adeno-associated virus (AAV) viral particles, as well as other types of viral particles (e.g., Adenovirus, FIV).

The VVC produces high-titer lenitviral and AAV viral particles at lower prices compared to commercial sources. Furthermore, using the VVC will likely provide a significant cost savings versus an individual lab trying to produce the viral particles on its own (consider the costs of reagent, labor, time, and troubleshooting).

The VVC has stock viruses (e.g., GFP-, mCherry-, and LacZ-expressing lentiviral and AAV particles) that can be purchased inexpensively by the vial. For generating custom viral particles, the investigator provides the VVC with a high-quality maxiprep of the transfer plasmid to be packaged into the viral particle of choice. Turn-around time is about 2 weeks for lentivirus and AAV viral particles.

 

You must have IBC approval to obtain viral particles.

   

This is often the rate-limiting step in obtaining viral particles, and we are not legally allowed to give out viral particles without this. The approval form can be found on the University of Miami Institutional Biosafety Committee website.

 

 

 

VIRAL VECTOR OFFERINGS

Price
(UM)

Price
(outside UM)

Lentivirus production (Full prep)

 $950

 $1482

Lentivirus production (Half prep)

$475

$737

AAV particle production (FPLC-purification )

$1950

 $2958

Aliquots of Stock AAV (10uL/vial)

$101

$149

Aliquots of Stock Lenti (20uL/vial)

$48

$76

Maxiprep plasmid preparation

 $225

 $342

 

 

ORDER FORMS


VVC Core Stocks Viral Particles Order Form


VVC Custom Viral Particles Order Form

 

 

 

 

PLASMIDS

 

VVC Maps and Sequences

 

 

LAB STAFF

VVC Manager

Pingping Jia,M.S. 

Phone: 305-243-0407

Scientific
Core Director

Vance Lemmon, Ph.D.
 Phone:  305-243-6793

 

 

 
 
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